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Y-STR DNA Explained Through a Real Cold Case #truecrimeshorts #truecrimeinvestigation #crimejunkies

May 23, 2026 / 01:41

This episode discusses YSTR DNA testing, the 1991 yogurt shop murders in Austin, Texas, and the connection to serial killer Robert Eugene Brashers.

The episode begins with an explanation of YSTR DNA testing, which focuses on the Y chromosome found only in men. This testing is useful in cases with mixed DNA, such as sexual assault kits.

A significant case highlighted is the 1991 yogurt shop murders, where YSTR testing was newly introduced. Investigators believed one victim had been sexually assaulted, leading to the extraction of a partial male DNA profile.

Initially, the DNA did not match anyone in the system, and the case went cold for decades. However, in 2025, a South Carolina lab linked the yogurt shop profile to Robert Eugene Brashers, a serial rapist and killer.

Brashers had died in 1999, but the evidence allowed investigators to connect him to the yogurt shop murders, providing closure to a case that remained unresolved for over 30 years.

TLDR

YSTR DNA testing links 1991 yogurt shop murders to serial killer Robert Eugene Brashers.

Episode

1:41
00:00:00
Number two, YSTR DNA. Now, this type of testing zeros in on one thing and one thing only, the Y
00:00:08
chromosome, which only exists in men. So, it can only trace DNA passed down from father to son. This type of testing
00:00:15
can be particularly powerful when evidence has a mix of male and female DNA, like for example, what could be
00:00:22
present in a sexual assault kit. By isolating the Y chromosome, labs can pull out just the male DNA from the
00:00:30
sample. One chilling example of this? The 1991 yogurt shop murders in Austin, Texas. [music]
00:00:36
At the time, YSTR testing was brand new, and the district attorney overseeing the
00:00:42
case decided to give it a shot. Investigators believed that at least one of the victims in this case had been
00:00:48
[music] sexually assaulted, and this test paid off. They pulled a partial male DNA profile from the evidence.
00:00:55
>> [music] >> But, in the '90s, that profile didn't match anyone in the system, so the case
00:01:00
went cold. Decades later, in 2025, everything changed when police received a report from a South Carolina state lab
00:01:07
that linked the yogurt shop unknown YSTR profile to a known YSTR profile from a 1990 sexual assault and murder out of
00:01:17
Greenville. The suspected killer? Robert Eugene Brashers, a known serial rapist and killer who had taken his own
00:01:25
life back in 1999. From there, investigators were able to use other evidence to connect Brashers
00:01:31
to the yogurt shop murders, bringing long-awaited answers to a case that haunted Texas and us crime junkies for
00:01:38
more than 30 years.

Badges

This episode stands out for the following:

  • 85
    Biggest twist
  • 80
    Most shocking
  • 80
    Most surprising
  • 75
    Most intense

Episode Highlights

  • YSTR DNA Testing Explained
    YSTR DNA testing focuses solely on the Y chromosome, tracing male lineage from father to son.
    “This type of testing zeros in on one thing and one thing only.”
    @ 00m 03s
    May 23, 2026
  • The Yogurt Shop Murders Case
    Decades after going cold, DNA evidence links a known killer to the 1991 yogurt shop murders.
    “Bringing long-awaited answers to a case that haunted Texas for more than 30 years.”
    @ 01m 35s
    May 23, 2026

Episode Quotes

  • This type of testing zeros in on one thing and one thing only.
    Y-STR DNA Explained Through a Real Cold Case #truecrimeshorts #truecrimeinvestigation #crimejunkies
  • The suspected killer? Robert Eugene Brashers.
    Y-STR DNA Explained Through a Real Cold Case #truecrimeshorts #truecrimeinvestigation #crimejunkies
  • Bringing long-awaited answers to a case that haunted Texas for more than 30 years.
    Y-STR DNA Explained Through a Real Cold Case #truecrimeshorts #truecrimeinvestigation #crimejunkies

Key Moments

  • YSTR Testing00:03
  • Yogurt Shop Murders00:32
  • Cold Case Solved01:35